Size and buffer

Size : 0.5µg

Buffer : 10mM Tris-HCl(pH7.4) , 1mM EDTA

 

 

How to use

1. Introduce pSuperAgro into competent cells of Agrobacterium by electroporation and select transformed Agrobacterium by gentamycin.

2. Make competent cells of the transformed Agrobacterium at step 1 and introduce your binary vector by electroporation.  Select them by antibiotics.

3. Infect Agrobacterium which is obtained at step 2.to plants.

 

※We recommend selection of Agrobacterium by gentamycin at 50µg/mL for agar plate, 20µg/mL for liquid medium.

 

 

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